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Eighty-three Salmonella isolates of 5 different serotypes, Newport, Saint Paul, Montevideo, Typhimurium, and Reading, were collected from 72 stranded seals; Newport was the most common serotype.
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Before each measurement, the stage of the optics module of the spectrometer was cleaned with 100%% ethanol and allowed to dry, and a new background reading was collected.
A total of more than 120 million 150 bp paired-end reads were collected by sequencing of transcripts of triplicate samples at each developmental stage.
In total, 234.7 Gb of Illumina readseq reads were collected from 31 samples at various development and physiological stages (Supplementary Data 2).
All sequencing reads were collected with standard Sanger sequencing protocols on ABI 3730XL automated sequencers at the Joint Genome Institute, Walnut Creek, CA. Three different sized libraries were used for the plasmid subclone sequencing process and paired-end sequencing.
Unmapped paired end reads were collected and mapped upon the genomic scaffolds of unmapped sequences from WAB56 104 using BWA mem with the default condition.
6073272 36 bp reads were collected with an average insert length of 400 bp.
Approximately 360 M paired-end reads were collected per lane.
Finally, all mapped reads were collected for further analysis.
The mapped reads were collected and both orientation R1 and R2 were retrieved.
In total, 189,819,468 reads were collected for 384 individuals, among which 365 had reliable phenotypic data.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com