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The reading wavelength was set to 265.9 nm.
Addition of 2N Sulphuric acid (H2SO4) of a volume of 100 μL was performed thereafter before subjecting to ELISA reader (Tecan, Sunrise, Melbourne, Australia) for result observation with a reading wavelength of 450nm and a reference wavelength of 650 nm.
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The plates were read at wavelength of 490 nm using a microplate reader (Bioteck Powerwave™, USA).
The plates were read at wavelength of 490 nm using a microplate reader (Bioteck PowerwaveTM, USA).
The plates were developed using tetramethyl benzidine (TMB; Sigma), the colour reaction was stopped with 1 M sulphuric acid and the absorbance read at wavelength 450 650 nM.
The absorbance was read at wavelength of 420 nm.
The absorbance was read at wavelength 405 nm.
Plates were developed by the addition of a substrate and read at wavelength 405 nm.
Plates were read at wavelength 405 nm after 20 minutes of incubation.
After incubation for 0, 248 48, and 72 hours, optical densities were read at wavelength 570 nm.
Plates were read at wavelength of 540 nm on a plate reader.
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