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The reading was obtained at 507 nm.
The electrode was left in the sample until a stable reading was obtained.
Each patient was monitored by one investigator until a consistent 3-min ETCO2 reading was obtained using a capnometer built into a Datascope® cardiac monitor (manufactured in the USA).
The highest possible absorbance reading was obtained at a mole fraction of 0.67 for the reagent solution, and the absorbance was found to decrease at lower or higher mole fractions.
After 45 min, a baseline reading was obtained on a Bio-Tek FL600 fluorescent plate reader with an excitation at 490 nm and emission at 520 nm.
Heterozygous reading was obtained from genomic DNA sequence of the patient N1 (∼24 Kb) in the SNPs rs4331 (exon 15), rs4353 (exon 20), rs4359 (intron 23), rs4362 (exon 24) and rs4363 (intron 25).
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The de re reading is obtained in a different way, see Section 2.5.
Data on the mapping of individual reads was obtained using the 'pileup' option of MAQ.
A minimum of 60,000,000 sequencing reads was obtained per subject.
An average of 56,898,937 reads was obtained per lane.
The lowest number of reads was obtained at 49°C.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com