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Firstly, a space reading was conducted with each student.
Thereafter, a 2 × 2 × 5 repeated measures ANOVA (or mixed between within-subjects ANOVA) of the effect of diagnostic group (HC/BD) and sex (male/female) on body language reading was conducted.
Scar reading was conducted by 4 medical doctors of whom one (SD) examined 78% of the participants.
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Sorting of sequence reads based on index sequences and the subsequent extraction of sequence tags from reads was conducted using a script written in Perl.
Quality control of reads was conducted using FastQC (Andrews 2010).
Assembly of the expressed short reads was conducted in several ways.
Illumina sequencing, with 100-bp paired-end reads, was conducted on a HiSeq 2000 (Illumina).
Bioinformatic analysis of trimmed RNA-seq reads was conducted using the Tuxedo Suite [ 68].
SNP calling from MethylC-Seq reads was conducted using the Bis-SNP package (http://epigenome.usc.edu/publicationdata/bissnp2011/).usc.edu/publicationdata/bissnp2011/
In order to make them useful resources for SNP identification, de novo assembly of these remaining reads was conducted.
Assembling of 454 reads was conducted using two different computer programs Mira [ 15] and Newbler (Roche, Switzerland).
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