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The CV for reading variation was 34%.
This includes biologic and reading variation.
Analytical variation or reading variation was assessed by reading one scan of each subject twice by one observer.
This approach might also have been associated with errors due to imprecision in the reading, although three independent persons read the curves to reduce the reading variation.
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Thus, it is necessary to investigate the manner of diode reading variations at different directions of externally wedged fields.
If you google around for articles about Ashley Madison, the online dating site for married people, you'll usually end up reading variations on one of two pieces.
Due to every cycle read, variation of HRS/LRS with cycle-to-cycle is observed, which is slight read disturb.
If FVF was minor, read variation was likely a sequencing or mapping error and if it was near 0.5, read variation was likely caused by SNP.
To remove false positives and lower the search space for the combinatorial optimization step, we perform several read, variation and allele filtering steps with the details below.
The read variation number observed in the current study has been commonly associated with the Illumina platform as different lanes can produce different number of counts [ 25, 27].
At the 0.1% level of overall spike-in, cross-contamination down to 1% could be reliably detected above the background contamination and sample-to-sample SASI read variation, within the experiment.
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