"No one wants to read fifteen robustness tests in the main text, but someone who's really interested in replicating the results will" look at the appendix, Fanelli adds.
Our first step was to test the assumptions underlying HMMSplicer's algorithm by evaluating performance relative to key parameters: the required read length, the robustness of the HMM, and the ability to match the second piece of a read.
Technically, the NGS panel demonstrated excellent results for coverage, read depth, and robustness for all genes in all 250 patients and controls, except one control with poor DNA quality.
We found that Diversity profiles were qualitatively and quantitatively robust across the entire sampling range (from 10%% sampling onward; Additional file 6) whereas in case of qualitative robustness, Evenness had to rely on higher percentages of reads (>90 %) to reach robustness to technological undersampling (Additional file 7) [ 9].
Using simulations, we evaluated the robustness of deML read assignments for datasets at various error rates.
The third aimed to assess further the robustness of IUTA to differences of read coverage among samples.
While results from UProC and RAPSearch read assignments yielded a high prediction quality and robustness, UProC was much faster compared to RAPSearch.
To ensure robustness of findings, all 3 researchers read all the interviews and any ideas about emerging themes were discussed in detail until a consensus was reached.
One way to read our results is as a kind of "robustness test" of the conventional median voter theory which assumes that the median voter is strictly self-interested.
Subsequent discoveries of additional thermostable polymerases and the mutation of other enzymes in the polymerase family has enabled the development of high fidelity, long read polymerases, all of which have added to the robustness of the PCR technique.
