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Clearly, careful standardization of the reading methodology is a critical need.
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Sequencing was done on an Illumina HiSeq machine using 100 cycles and the paired-end read methodology to generate 100 base pair reads, as described by the manufacturer (Illumina, Inc., San Diego, USA).
Tag-based short sequence reads methodologies like SAGE (serial analysis of gene expression) or MPSS (massive parallel signature sequence) are also high-throughput and quantitative, however the sequence reads cannot be unambiguously mapped to the reference genome; the transcriptome is still partially analyzed; and it is usually not possible to distinguish different gene isoforms [ 18, 19].
For more details on the methodology used for the Guardian University Guide tables, please read our methodology guide.
One principal investigator (PS) was experienced in the study methodology; however, neither read nor spoke Chinese.
You can read her methodology, results and conclusions for yourself,¹ but the most striking result of her study: "When compared to the approximately 10percentt gender gap in peak running speeds of humans, the difference in the animals was small — no greater than 1.2percentt.
You can read his methodology in the document embedded below.
Read our methodology.
We applied the read depth methodology [ 26, 34, 35] to estimate CNVRs.
We applied the WSSD strategy [ 23] to the PN40024 grapevine genome to detect SDs (>= 10 kb in length, > 94% sequence identity) based on a read depth methodology.
On the basis of these data we cannot formally rule out the possibility that the true causative variant is a noncoding regulatory variant or that the true causative variant was entirely missed by the chosen short read resequencing methodology in combination with an incomplete reference genome.
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