Many of these strategies produce relatively short reads, in the range of 36 70 nt [5], compared to traditional Sanger sequencing which routinely produces reads >800 nt in length [6], [7].
We therefore expect to see increasing development of technologies that generate sequence reads in the range of many thousands of bases to facilitate haplotype assembly.
Mapped reads in the range of 20 million, as is the case for the postlarval sample, allow a close estimate of expression level for approximately 70% of low abundance genes [ 31].
These probability values give at each read location the probabilities of the base to be either A, C, G or T. Q bs (i): the probability of the base bs, in the form of a Q-value, at location i in a read in the range of [− Qmax, Qmax].
The significance of a blood pressure reading in the pre-hypertensive range may therefore be unclear.
In the genome browser, the reads track has a scrollbar on the left side for a user to visualize all reads in the chromosome range.
To extend a seed rseed at the 3′-end, SWA searches all unassembled unique reads in table R for extendable reads in the dynamic range of [1, k-mer], that is, from maximal overlapping to minimal overlapping.
To identify novel and non-annotated miRNA orthologs, we then sorted for the most abundant (>50 read counts) mapped reads in the size range of 19−24 bp across all nine libraries and filtered out all the annotated miRNAs.
The original RS system with C1 chemistry generated mean read-lengths in the range of 1,500 bp and yielded approximately 100 Mb of sequence data per run, and reads in this range were useful in generating improved scaffolds for de novo assemblies.
Speed is obviously of great importance, but equally important is maintaining alignment accuracy of short reads, in the 25 100 base range, in the presence of errors and true biological variation.
