Note that any instant-read thermometer will work, but a digital thermometer is probably your best bet since it allows you to read temperature fluctuations more precisely.
The remaining patterns of segregation may represent male recombination, but are also likely to include patterns that are artefacts due to sequencing errors or false negative allele nulls due to read coverage fluctuation for example.
The term was a hedge, an acknowledgment that without sustained firsthand access to a person — even, say, a ubiquitous billionaire celebrity whose teen-age pregnancy, sexual abuse, spiritual evolution, eighteen-year engagement, weight fluctuations, reading tastes, aching "vajayjay," and fondness for candlelit baths are familiar to millions — there's only so much that we can really know.
I tracked down a podcast on which a TSD researcher named Ido Pen talked about his research, and I transcribed his main point for your reading pleasure: "If the fluctuations between years are very large, that will select against temperature dependent sex determination.
I tracked down a podcast on which a TSD researcher named Ido Pen talked about his research and I transcribed his main point for your reading pleasure: "If the fluctuations between years are very large, that will select against temperature dependent sex determination.
Each value included in the analysis was the average of two technical repeats to protect against slight fluctuations in reading and small pipetting errors.
Our results suggest that fluctuations in read yield and sequence quality are more likely driven by individual run performance than by input template amount.
At low counts, Poisson variance is high relative to the total read count, and Poisson fluctuations can cause large fold changes (Additional File 1, Figure S2).
These fluctuations in reads across ORFs and a general increase in coverage around the 5' ends of ORFs are consistent with other RNA-seq datasets [ 28, 37- 39].
This difference may be due to statistical fluctuations of read sampling in the downsampling process, combined with the much smaller size of the chr12 capture region.
