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Relating objects from both SR images and their blurred versions allowed direct reading and quantification of cluster information per groups of molecules.
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Alignment of sequence reads and quantification of expression levels was performed with TopHat and Cufflinks [Trapnell et al., 2012].
Alignment and quantification of reads revealed high levels of transcription for subsets of both OBPs and CSPs.
The exposed screens were read in a Typhoon scanner and quantification of signal in particular brain regions was performed using ImageQuant software (both Molecular Dynamics Sunnyvale, CA, USA).
Approximately, 65 75% of the sequencing primer was extended in the reverse transcription reactions, based on CE detection and quantification of 'null ligation' reads (consisting of the second sequencing adapter ligated directly to the sequencing primer) after paired-end sequencing.
We used StringTie (version 1.2.1 26 for identification and quantification of transcripts from the aligned RNA-Seq reads.
The accurate reconstruction of transcript isoforms from short reads (such as provided by the Illumina platform) remains a technical challenge43, Thus, the identification and quantification of alternative splicing events may not be fully complete or reliable.
assisted with protein purification and quantification of protein yields.
Mapping and quantification of dopamine D2 receptor activation.
N.A. helped with HSC imaging and quantification of HSC distributions.
Intracellular TNF (left) and quantification of TNF producing cells (right).
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