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The first reading allows for the response that we should keep a closer watch on our politicians.
The process of naive reading allows for an overall grasp of the text as a whole, which gives an initial and holistic understanding of what the text contains.
Tagging of the chromosomal host DNA and the episomal 'survival plasmid' with different GFP variants compatible for double-label fluorescence reading allows for the normalised quantitative detection of the episomal plasmid.
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The tagging of each cell line by a distinct GFP mutant (capable of double-label reading) allowed for multiple time-point assessments of the relative cell viability through GFP fluorescence monitoring.
Such an index read allows for multiple samples to be sequenced in one lane (multiplexing).
However, since the introduction of 454+ a substantial fraction of the respective reads allows for a genus level assignment, and this situation is expected to even improve with future increases in pyrosequencing read length.
It is possible that, to some extent, the longer length of MiSeq reads allows for more opportunity for mapping of non-perfect matches, and this may contribute to a decreased LoD for MiSeq when mapping to a closely related but not identical reference sequence.
Detection of SNVs and small InDels by these two different platforms demonstrated its complementary power; whereas Illumina GA provides a significant depth (67× in this case) useful for resolving many SNVs and small InDels, the length of 454 reads allows for the detection of these GVs in highly polymorphic regions.
Importantly, they use extremely short (25 bp) reads, allowing for larger number of fragments at equal coverage depth.
The problem is exacerbated with longer reads: allowing for one mismatch per read is acceptable for 35 bp reads, but insufficient for 100 bp reads.
For each read, candidate alignment locations are identified with a hash lookup of the 15-mers in the read, allowing for one mismatch.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com