Their attempts to understand the nature of their condition, how such cruel behaviour can be so readily validated, give the reader a much-needed opportunity to seek enlightenment too.
Furthermore, the crack opening displacements are physically more meaningful and readily validated against the finite element analysis predictions.
It was determined from our previous microarray analysis of gene expression in DIO B6 mice that the application of false discovery rate (FDR) corrections caused a failure in the ability to identify genes associated with variations in DIO that can be readily validated via qRT-PCR [21].
Simple biomarkers of response are favored over complex expression signatures because they are familiar in toxicology assessment, are easy to maintain over time (e.g., are independent of the microarray platform), and can be readily validated.
The benefit of using real-time qRT-PCR for cancer diagnostics is that new markers can be readily validated and implemented, making tests expandable and/or tailored to the individual.
In addition to validated assays currently available and used by the U.S. EPA Endocrine Disruptor Screening Program (EDSP) (U.S. EPA 2011), U.S. Food and Drug Administration FDAA), NTP, Organisation for Economic Co-operation and DevelOECDnt (orCD), otherhestablishedhed governmental programs, we also included those that could be readily validated in the future.
To characterise and compare the global proteomic alterations prompted by KSR1 in the presence of doxorubicin or etoposide, we conducted a triple-labelling SILAC strategy coupled to high-resolution quantitative MS as readily validated in our two recent studies [ 15, 27].
For intron inclusion, both junction-spanning and read-abundance-based reads are useful and a variant can readily be validated with either, provided that the variant-containing experimental sample(s) show a statistically significant increase in the presence of either form of intron inclusion corroborating reads.
The protocol established may be readily applied to validate future IBA software as well.
Insights gained during the study will help to develop and, in a second study phase, to validate readily applicable diagnostic algorithms that shall facilitate the clinical decision-making and management of patients presenting with persistent diarrhoea and abdominal pain.
