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Cell suspension was readily mixed with 1.5 ml ethanol 96% at -20°C, and the final mixture was kept in ice during 15 min to allow cell fixation.
Hence, the recommended readily mixed consistency of the Calcicur paste was used for the Syringe-Lentulospiral and Syringe-Spreader methods.
When 170 g/l of PSOM and 35 FPU/g PSOM were used, the culture could not be readily mixed.
This suggests that kin discrimination did not play an important role in our experiment as genetically different strains readily mixed and formed chimeric fruiting bodies when given the opportunity.
The most basic nature of the MMV technology can be represented by the facts that the liquid in an MMV well does not fall by the gravity and is less volatile due to the narrow aperture area, easily thermo-conductive due to the small volume, and readily mixed by convection.
It's easiest to buy readily mixed media suitable for the orchid type, and always know the specific needs of the variety of orchid you're growing, as these can vary quite a bit.
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Good sugar yields were obtained with all the samples, demonstrating that SO2-catalyzed steam pretreatment is a robust and efficient fractionation process that can readily process mixed feedstocks.
The oligopeptide-carrying phospholipid derivatives were readily incorporated into mixed micelles consisting of natural phospholipid (dipalmitoyl phosphatidylcholine, DPPC) and Triton X-100 as surfactant.
They have the advantage, however, that all problems of system analysis (like controllability, observability, stability and verification) and all problems of synthesis (like controller design and filter design) can be readily expressed as mixed integer linear or quadratic programs, for which many commercial software packages exist.
Without PMA treatment, C t values decreased as the proportion of dead R508N cells increased in the mixture, demonstrating the ability of DNA from compromised/damaged cells to be readily amplified when mixed with DNA from live cells.
Following co-culture and leukemia-inhibitory factor (LIF) withdrawal, Jarid2-null E8-derived (GFP) cells expressing neural-associated markers such as Nestin and Mash1 (red) were readily identified within mixed cultures (47% of GFP+ cells expressed Nestin).
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