Suggestions(1)
Exact(4)
As the authors point out, it is well understood that PCNA does not readily load on to DNA on its own, and that once loaded it is very stable on DNA because it forms a closed ring.
10.7554/eLife.01213.010 Figure 4. Engineering an analogue of the carbamylated lysine residue, Cx26K125E, creates a constitutively open hemichannel that no longer responds to CO2. (A ) HeLa cells expressing Cx26K125E readily load with dye under control conditions.
Under conditions of normal extracellular Ca2+, HeLa cells expressing wild type Cx26 hemichannels readily load with carboxyfluorescein when exposed to a moderately hypercapnic saline (PCO2 55 mmHg) (Huckstepp et al., 2010a; Meigh et al., 2013).
10.7554/eLife.01213.012 Figure 5. Bridging in the reverse direction: the mutation R104E forms a salt bridge with K125 in Cx26R104E to create a constitutively open hemichannel that no longer responds to CO2. (A ) HeLa cells expressing Cx26R104E readily load with dye under control conditions.
Similar(56)
Doxorubicin (DOX) was then readily loaded into the hydrogels and the in vitro release profiles indicated that the release rate increased rapidly while pH decreased from 7.4 to 5.0, which is consistent with the degradation rate of these hydrogels at corresponding pH conditions.
PTX can be readily loaded into PEG5K-S-S-FTS2 micelles.
We found that HeLa cells expressing Cx26K125E readily loaded with dye under control conditions and exhibited no sensitivity to CO2.
In the case of AspGTC, the isoform (29mer) that is most abundant was not detected at all in Ago1, while it was readily loaded into Ago2, which also showed increased loading with age (Fig. 2c-d).
As expected from our previous work, HeLa cells expressing the Cx26 readily loaded with CBF when exposed to this dye in the presence of elevated PCO2 (55 mmHg, at pH 7.5, Figure 1A,B).
Although MPLA can be readily loaded in the membranes of the ICMV capsules, we previously found that simply admixing this adjuvant with ICMVs gave strong humoral responses, and thus we used this approach of simply admixing MPLA and ICMVs to keep the formulations as simple as possible.
The Young's modulus of MWNTs (a measure of their elasticity, or ability to recover from stretching or compression) is estimated by researchers to be greater than that of carbon fibres by a factor of 5 to 10. MWNTs are capable of readily absorbing loads via a sequence of reversible elastic deformations, such as buckling or kinking, in which the bonds between carbon atoms remain intact.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com