The spots corresponding to the number of IFN- γ, IL-4, IL-6, and IL-10-secreting cells were counted automatically with a CTL ELISPOT reader and analyzed using the ImmunoSpot image analyzer software v4.0.
Absorbance was measured using a BioTek ELx800 plate reader and analyzed using the KC4 data analysis software (BioTek, Winooski, VT).
Plates were read at 450 nm with an ELISA-reader and analyzed with KC4 3.03 Rev 4 software.
After 15 30 min of incubation, reaction solutions were stopped with 2 M NaOH and read at 405 nm using a µQuant microplate reader and analyzed with Gen5 software (BioTek, Winooski, VT).
Absorbance at 450 nm was measured using TECAN Infinite® M200 microplate reader and analyzed using Magellan™ software.
Absorbance at 450 nm was measured using a TECAN Infinite® M200 microplate reader and analyzed using Magellan™ software.
This system uses SoftMax Pro (version 2.6.1; Molecular Devices) software to run the plate reader and analyze the data.
The fluorescent intensity of the beads is detected by the Illumina BeadArray Reader and analyzed using Illumina BeadStudio software.
Optical densities were measured at 450 nm using a microplate reader and analyzed with Gen5 Data Analysis Software (Bio-Tek, Winooski, VT).
Optical densities were read at 450/595 nm on a Spectramax190 microplate reader and analyzed using SoftMax Pro software (Molecular Devices).
