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A reader was determined to have successfully located a real lesion (malignant or benign) if he or she marked a finding in a quadrant matching the true location of the lesion and described a finding type consistent with the true finding type.
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Agreement between the two readers was determined using Cohen's kappa, with κ values of 0 indicating poor agreement, 0.01 to 0.2 slight agreement, 0.21 to 0.40 fair agreement, 0.41 to 0.60 moderate agreement, 0.61 to 0.80 good agreement, and 0.81 to 1 excellent agreement [31].
Agreement between readers was determined using κ statistics.
The statistical significance of differences in sensitivity, specificity and accuracy between the two levels of routine readers, was determined by generalized score tests trough marginal models (link logit).
Inter-observer agreement for visibility between the two readers was determined by calculating Cohen's kappa using a weighted kappa test (poor agreement, ĸ = 0; slight agreement, ĸ = 0.01−0.2; fair agreement, ĸ = 0.21−0.4; moderate agreement, ĸ = 0.41−0.6; good agreement, ĸ = 0.61−0.8; and excellent agreement, ĸ = 0.81 1).
Using an enhanced formulation of the maximal covering location problem along with a new criticality index analysis metric (derived from the severity, frequency and dwell time of the critical medical assets) the optimal placement of the limited number of RFID readers is determined.
Inter-reader agreement was determined using Cohen's kappa coefficient.
The absorbance was measured at 540 nm on the Bio-Rad Benchmark Plus Microplate Reader and the reaction rate was determined using the formaldehyde standard curve.
Absorbance at wavelengths between 420 and 480 nm was then measured with a microplate reader and WST-1 activity was determined to evaluate chemosensitivity.
Spectrophotometric absorbance of sample was determined by microplate reader (Biorad) at 570/630 nm.
Absorbance of the supernatants at 540 nm was measured with an ELISA plate reader, and 0 and 100% hemolysis was determined in PS and 0.1% Triton X-100, respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com