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Figure 3 Photograph and schematic of the hybrid RFID/UWB reader measurement setup used for decoding of the chips' transmitted UWB sequence.
The intra-assay coefficient of variation for repeated AF reader measurement on the same day was 5.0% (Meerwaldt et al. 2004).
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The high temporal resolution of the plate reader measurements allows us to obtain a measure of the change in promoter activity and growth rate as a function of time.
The data obtained from the plate reader measurements were analyzed with a custom Matlab (MathWorks) program.
A polyolefin film cover was used to cover the plate, and plate reader measurements were begun immediately.
For plate reader measurements, the wells were filled with 50 μL enzyme solution, 12.5 μL inhibitor solution, and 50 μL SKF solution in the given order.
This bias would likely not impact the ranking of individuals by renal sinus fat accumulation within each set of reader measurements.
We have introduced a new method to quantify protein expression that uses plate reader measurements of emissions at two wavelengths and spectral unmixing to correct for autofluorescence.
For intracellular Ca2+ measurements cells were seeded at confluence on glass coverslips (for confocal imaging analysis) or on 96-well essay plates (Corning, CellBIND surface, Tewksbury, MA, USA, for multiplate reader measurements).
Similarly, for Flexstation multiplate reader measurements (Flexstation 3, Molecular Devices, Sunnyvale, CA, USA), the cells were loaded with Fura-2-AM and de-esterified for 20 min at 37 °C.
Here we propose a general method for correcting plate reader measurements of fluorescent proteins that uses spectral unmixing and determines both the fluorescence per cell and the errors on that fluorescence.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com