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The sample is added to the cartridge, which is then placed in the reader instrument.
In parallel, when starting above staining, we deposited 40 μL of whole blood on an Accellix cartridge that was immediately placed in the reader instrument (Fig. 1).
More compelling is the potential of the NINA heater as the core component of a stand-alone assay kit, capable of providing a result without external electrical power, a reader instrument, or any complex ancillaries.
Slides were scanned by a BeadXpress Reader instrument (Illumina) and data analysed by the Bead Studio Methylation Module Software (Illumina).
Fluorescence emission at 590 nm from the wells was detected via excitation at 530 nm in a multiwell fluorescence plate reader instrument (CytoFluor II, PerSeptive Biosystems).
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The microplate was read by using an ELISA-reader instrument (Tecan Infinite M200) equipped with Magellan V6.55 software.
The increase in absorbance at 405 nm was measured by microplate reader (BioTek Instrument, Seoul, Republic of Korea) to analyze the release of pNP.
The amount of colored formazan was determined by its absorbance at 570 nm in a BioTek ELISA Microplate Reader (BioTek Instrument, Inc., Winooski, VT, USA).
Samples were read in a spectrophotometer (ELx800 Absorbance Microplate Reader, BioTek Instrument, Luzen, Switzerland).
Then intensity of colour was measured at 450 nm using spectra count ELISA reader [Packard Instrument Co., Downers Grove, IL, USA].
Plates were read at 450 nm using a Synergy HT microplate reader (Biotek Instrument, Winooski, VT, USA).
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