Somehow the New Yorker's arcane use of a diaeresis (coöperation) or acute accent (élite) seems quaint - and doesn't interrupt a reader's flow like an additional, superfluous word; even e-mail doesn't itch.
To measure the level of cellular oxidative stress, cultures were subjected to DHE staining at indicated time points, followed by quantification using a fluorescence reader or flow cytometry as previously described.
Additionally, simple equipment can be used to the perform analysis using this reporter, in contrast to the expression profiles of various libraries based on fluorescent proteins, which require analysis using fluorescent plate readers or flow cytometry.
Generally, in the elementary process, the RFID tag reader triggers information flow through transmitting a query signal.
Oxidative stress was determined by measuring the conversion of dihydroethidium (DHE, Sigma-Aldrich) to the red fluorescent ethidium applying a fluorescence plate reader or a flow cytometer.
Then, the optical density of each culture well was measured using a microplate reader (Titertek Multiskan, Flow Laboratories, McLean, VA, USA) at 590 nm.
To address the concern, we have added a statement to the Introduction preparing the reader to the flow of the logic that follows.
The review highlights the merits of each program or database and it provides the reader with a flow chart (their figure four) to assist in the best choice of tool for a particular application.
All of us readers know that flow state when we read,we don't think about the glue, the paper, the stitching, allof that goes away.
