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For plate reader experiments, biological replicates consisted of cells inoculated into different wells in a 96-well plate that were exposed to equivalent inducer concentrations.
For plate reader experiments, cells were grown at 37 °C for ∼6 8 h, and then diluted to OD600 of 0.01 in a 96-well plate (Corning) in LB Lennox media (Sigma).
However, this is recognized as a relatively low number for plate reader experiments.
> -wrap-foot> aCofcenthetinhibitore inhibitor was 1 μM TAK715 (final concentration) in the plate reader experiments.
Data assessment: The results of plate reader experiments were processed in Microsoft Excel and Origin 6.0 for fitting the calibration.
Black-bottomed PP-96-well microtiter plates from Greiner Bio-one (Alphen a/d Rijn, The Netherlands) were used for all microplate reader experiments.
Similar(52)
The BBC conducted various trials during the year, including mobile radio and TV; community-orientated projects on Second Life, Flickr and YouTube; a catch-up service for personal video recorders; a barcode reader experiment at London Zoo and a podcast for disabled users.
Therefore, an additional plate reader experiment with n = 50 was performed, yielding a Z′ factor of −0.02.
You can always include a Future Directions section that would state for the reader the experiments you would propose next!
This perspective evaluates different methodologies for identifying and reducing the contribution of multiple molecule interactions to single-molecule measurements to help the reader design experiments or assess publications in the single-molecule force spectroscopy field.
Furthermore, with the aim of not only validating the algorithm and the software, but also demonstrating how the tool is to be used, we provide the reader reproducible experiments that demonstrate the capability of the proposed segmentation tool on several public available data sets.
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CEO of Professional Science Editing for Scientists @ prosciediting.com