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The high-content screening instrument programmed to detect and quantify fluorescent spots enabled the automatic imaging of ß-DG fluorescent clusters on a Cellomics ArrayScan VTI HCS Reader and analysis by the Compartmental Analysis BioApplication.
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The optimal method should balance validity, ease of interpretability for readers, and analysis expertise of the research team.
The best stories provide the reader with context and analysis: they explain how the news fits in with what has gone before and how it affects our understanding of the subject, our society or environment, the way policy is decided, or whatever.
The colour reaction was developed using TMB substrate (30 min at RT), stopped with 0.5 M H2SO4 (50 μL) and read at 450 nm (Tecan Sunrise ELISA plate reader and Magellan data analysis software).
Twenty microliters of the reagent 3- 4,5-dimethylthiazol-2-yl -5- 3-carboxymethoxyphenyl -2- 4-sulfo-phenyl -2 H-tetrazolium salt (MTS) in 100 μL fresh medium was added to each well; the cultures were returned to the incubator; and the absorbance at 490 nm was measured 1, 2, and 3- 4,5-dimethylthiazol-2-yl -5- 3-carboxymethoxyphenyl -2- 4-sulfo-phenyl -2cular Devices, Sunnyvale, CA).
The Observer takes the lead in breaking significant, agenda setting stories... Led by an award winning team of correspondents and photographers, Observer Sport aims to provide the reader with unrivalled reporting and analysis of both domestic and global events..
Using worked examples, this chapter takes the reader through the design and analysis of three heat pipes, one for a spacecraft application and two for standard units.
"Not a day goes by that we don't give our readers the latest news and analysis on the deepening troubles of the US economy," Hartenstein wrote.
The Los Angeles Times will also provide readers with news coverage and analysis in print and online.
An important limitation to this study was that the readers were not blinded and analysis was performed in a side-by-side comparison, thereby potentially introducing bias.
Kaveh Madani is a water management expert and reader of systems analysis and policy at the Centre for Environmental Policy of Imperial College, London.
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