The reads were filtered to remove low quality reads, yielding 2,705,634 reads with an average read length of 377 bp.
Ilic et al. also proved that empty capture sites and broken cells display lower number of total reads yielding a decreased number of detected genes.
The expense for sequencing with Sanger technology is about $500/Mbp (800 bp reads), whereas e.g. pyrosequencing with Roche's 454 technology is only about $100/Mbp (250 bp reads) yielding many more base pairs per run at the same time.
NGS of the whole transcriptome resulted in 19,534,766 sequence reads, yielding a total of 40,260 transcripts (N50 = 522 bp).
A total of 103,860 high quality reads, yielding 37,739,423 bp of genomic information, were retained for analysis.
We assembled the transcriptome of a clonal population of adult, aposymbiotic (dinoflagellate-free) Aiptasia pallida from ~208 million reads, yielding 58,018 contigs.
Two sequencing runs of each CWR and N-IWR cDNA libraries generated 306 million 50 bp reads yielding more than 15 gigabase of sequenced data.
In lodgepole pine, NGen was used to assemble a transcriptome from 0.6 million 454 reads, yielding 63,687 contigs with a mean length of 500 nt [ 22].
Not surprisingly, earlier de novo assemblies of transcriptomes of other non-model plants generally used fewer and shorter 454 reads, yielding fewer and shorter contigs [ 23, 29- 34].
The BRBS cDNA pool sequenced using the Roche 454 approach generated 2.53 million reads yielding a total of 857 Mb raw sequence data.
