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We compared the read distribution uniformity using 0.1X extracted data from all the YH single cells mentioned above.
Baseline plate reads with no added compound demonstrated robust signal and excellent well-to-well uniformity in 1,536-well 1,536-wellgure 1).
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Film read out consistency, response uniformity, film-scanner sensitivity, long term stability and total dose uncertainty was evaluated.
Hammer improves on Quake, retaining about 19K more genomic reads while reducing the number of erroneous reads by ~390 K. Though Quake was designed with uniformity in mind, an alternate method called HiTEC was recently published, which does not strongly rely on uniformity assumptions (Ilie et al., 2010).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com