Exact(22)
Therefore, it is necessary to develop multiple sequencing approaches and platforms, including the third-generation long read technologies, high-quality long-insert clones and new assembly algorithms.
For this work, we utilized first generation short read technologies, and read quality and quantity have dramatically improved thereafter.
This suggests it represents an effective filter for functional transcripts, in lieu of more reliable transcript quantification becoming available from the use of longer read technologies.
Thus, de novo assembly using 454 technology or a combination of 454 and shorter read technologies [ 16], followed by expression profiling using short reads seems a reasonable approach.
Instead of performing a two round nested PCR, as is required for shorter read technologies, the fusion transcripts were amplified in one single round.
Such sequences collapse into single contigs in WGS assemblies, particularly when data are obtained using the NGS short read technologies, and therefore biologically relevant information is lost.
Similar(38)
For pharmaceuticals, read technology.
I kept thinking as I read, "technology can change the world".
The challenge with de novo transcriptome sequencing using data from short read technology lies in the difficulty of assembling the reads into contigs reflecting transcriptional units [3].
Reads with more than one SNP are difficult to assemble when using short read technology.
Illumina HiSeq system 2x100 bp paired end read technology was used for genome sequencing.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com