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i-Tracker can read spectra in.dta or.mgf formats.
After three washes, the enzyme reaction was developed for 30 min and stopped with sodium hydroxide EDTA carbonate buffer, and plates were read (SPECTRA II; SLT Labinstruments, Grödig, Austria) at 550 nm.
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The microtiter plates containing the P450 samples were first used to read blank spectra at 450 and 490 nm using a Tecan Safire 2 plate reader.
The resulting disturbance changes the bonds of molecules, and the researchers can read the spectra to see which elements were bonded to which.
Microplates were read by Spectra-Max 190 Absorbance Microplate Reader (Molecular Devices, Inc., USA) and analyzed by SoftMax Pro (Molecular Devices, Inc .. Total cellular RNA from frozen muscle tissue and liver samples were extracted in TRIzol reagent (Invitrogen) and homogenized with FastPrep w-24 Lysing Matrix D (MP Biomedical) and Precellys w-24 (Bertin Technologies) for 15 s.
Manual validation of the phosphorylated peptides/proteins is, on the other hand, a good challenge, although it implies great expertise with "reading" the spectra [28 35].
The instrument was standardized with the equalizer sample before reading the spectra of the samples collected from each farm.
The fluorescence was read on a Spectra Max M4 multi-detection micro plate reader (Molecular devices, CA, USA) at 480-nm excitation/530-nm emission.
Microtiter plate was read using the Spectra Max Gemini EM reader (Molecular Devices).
The plates were read using the Spectra Max Gemini EM reader (Molecular Devices).
The absorbance was read on a Spectra MAX 340 at 405 nm (Molecular Devices, Sunnyvale, CA, USA).
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