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Short read sequencing technologies are appealing, as the cost per base is relatively cheap [ 6].
AT and AF carried out short read sequencing by using Illumina genome analyzer.
Single read sequencing yielded 18 and 32 million uniquely mapped reads for RXRα and RARα, respectively.
Thus, short read sequencing cannot capture unique sequence and define copy number.
PacBio technology uses a process called strobing to perform paired-end read sequencing.
Recently, several chloroplast genomes were published using long read sequencing technology from PacBio [ 20– 23].
Similar(36)
The cDNA library was sequenced using Illumina HiSEQ2500 sequencer with a one lane, 50 cycles, single-read sequencing strategy.
However, long-read sequencing is still costly, while short-read sequencing carries less information for complex microbial community profiling; therefore, the applications of high throughput sequencing platforms still remain challenging in microbial community reconstruction analysis.
Massively parallel, short-read sequencing of mRNA libraries was used to generate an extensive map of the transcriptome of the adult, murine neural retina.
Here, we present a de novo genome assembly from single-molecule, long-read sequencing data consisting of 135 P. pacificus contigs.
Briefly, this method involved a step of genome complexity reduction using PstI/TaqI restriction enzymes followed by Illumina short-read sequencing of the restricted products.
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