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This device was used to read results from an array of antibodies conjugated with either an enzymatic or fluorescent tag resulting in a colored or fluorescent readout.
Read results from 'Keitt' and 'Tommy Atkins' mRNA resequencing using Illumina HiSeq 2000 were mapped to the 'Keitt' reference-transcriptome contigs using bowtie2 (http://bowtie-bio.sourceforge.net/bowtie2/index.shtml).shtml
For some studies that did not include quantitative results in the text, we were able to read results from survival curves presented in figures.
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Any singleton read resulting from read trimming was removed [ 80].
Read pairs were then synchronized such that all read pairs with sequences of at least 35 bp on both sides after quality trimming were retained and any singleton read resulted from reads trimming were removed.
The software presented here offers a practical and accurate method to identify potential vaccine and diagnosis candidates against pathogenic bacteria by "reading" results from well-established reverse vaccinology software in a novel way, considering the epitope's concentration in the mature portion of the protein.
These events are relatively rare and considerable effort is needed to detect a small number of chimeric sequences amongst millions of non-chimeric cDNA reads resulting from a CLASH experiment.
This number divided by the total number of reads gave the estimated percent of correctly barcoded reads resulting from ligation.
The number of reads resulting from deep sequencing is often considered a reliable quantification of miRNA expression [19].
HSVd- and GYSVd1-sRNAs fulfilling these criteria were identified in all tissues, with the highest (about 135000 and 102500) and the lowest (about 5300 and 5800) reads resulting from tendril and leaf samples, respectively (Fig. 1A and 1B).
A total of 643168 single-end reads resulted from the GS-20 sequencer for S. simiae.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com