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Absorbance was measured at 412 nm every 9 seconds for 3 minutes on a SPECTRAmax PLUS 384 spectrophotometer, this constitutes the pre read plate.
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The plates were read plates at 450 nm within 30 min of adding the solution.
8. Read plates on ELISA reader at an absorbance of 492 nm.
According to Ms. Melander, the system reads plates accurately 93percentt of the time; plates that can't be read by the software are entered manually.
Cell proliferation was determined by reading plates at 450 nm.
In between reads, plates were stored at room temperature and protected from light.
The plate was read using plate reader (Synergy HT Multi-Mode Microplate Reader, Biotex, Houston, TX, USA) at 450 nm.
We then read the plate on a FluoStar plate reader (BMG Labtechnologies, Durham, NC) at 450 nm.
We read the plate in a 96 well plate reader at 550 nanomoles (Tecan, San Jose, CA, USA).
The device read the plate number as 5SOW750, but Green's car had a plate number 5SOW350.
The results are read by plate readers or cell cytometers.
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