Exact(4)
This is primarily due to repetitive sequences, but true variations and sequencing errors may also obscure where the read originated.
Therefore, the GMS uses simulated reads so that it can guarantee 100-fold coverage and know exactly where in the genome the read originated from.
These could, for example, be based on the frequency of k-mersof different classes, giving rise to the computation of the likelihood that a read originated from either the host or graft genomes.
The posterior probability that a read originated from one of the loci to which it aligns can be computed using an expectation-maximization (EM) algorithm (Li et al. 2010; Nicolae et al. 2011; Roberts and Pachter 2013; Patro et al. 2014).
Similar(56)
Among them there were Herbaspirillum spp. and Halomonas spp. These organisms were identified by binning the DNA reads originated from KMC grown in sterile and non-sterile media.
Of 1,787,490 mapped sRNA reads found in the virus-treated sample, 80.1% (1,431,362 reads) originated from Arabidopsis and the remaining 19.9% (356,128) of the reads originated from the virus (Figure S1, A and B).
74 75% of the sequence reads originated from the targeted region in the SureSelect libraries and 41 67% in the Nimblegen libraries.
We retained only aligned reads that overlapped by at least 4 nt to each side of the exon-exon junctions; this criteria was set to ensure that the reads originated from the junctions.
We found that a large fraction of Illumina 1 G reads originated from highly abundant ribosomal rRNAs.
At the DNA level, most nonhost reads originated from nonpathogenic single-stranded DNA anelloviruses (7 ).
In wild-type cells the majority of reads originated from two classes of retroposons.
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