Exact(5)
BAM sorting, duplicate read marking, and realignment to improve in/del discovery all occur at this step.
ClinSeK also implements a refined duplicate read marking algorithm that is aware of not only alignment positions but also base identities and qualities (Additional file 1).
The reads were cleaned by indel realignment, duplicate read marking and quality score recalibration, and SNP variants were called with a trio-aware caller.
BAM sorting, duplicate read marking, realignment, base quality recalibration, variant calling and filtering were performed with the same parameters used for the bwa-GATK variant calling pipeline in Proton reads.
Annotate as you read, marking specific reoccurring elements, motifs, metaphors, and anything relating to a theme that the author portrays.
Similar(55)
It is more important that it should be read, marked and inwardly digested by all three of the remaining candidates to succeed George W. Bush as president of the United States.
The Court of Appeal handed down a judgment yesterday that deserves to be read, marked, learned and inwardly digested by every British university vice-chancellor.
Changeux has read, marked, learned and inwardly digested Darwin's (1871) Descent of Man, which argues convincingly that human beings inherited their social insects from their social primate ancestors, which could not afford to act like egoists.
The voting machine that Appel and Jamieson picked to illustrate this hypothetical "man-in-the-middle" attack was the DS200, a popular optical-scan voting machine that reads marked paper ballots, made by a company called Election Systems & Software.
After quality based read trimming, sequence reads were aligned to the GRCh37/hg19 human reference genome using BWA [ 19] and duplicate reads marked using the Picard program [ 23].
For this purpose, all reads marked as singletons were mapped to the isotigs of the assembly using ssaha2 [ 60] with default parameter settings.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com