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Following a brief scene where Heydrich bitches out one of his collaborators, Alfred Naujocks, for recording him while having sex in a whorehouse built to gather blackmail evidence against members of the party, Binet admits that all the dialogue you've just read is constructed from an account written down by Naujocks himself, which Binet imagines couldn't be how it actually went.
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In the third phase, a multiple sequence alignment of reads is constructed and a consensus sequence along with a quality value for each base is computed for each contig.
The reference-based assemblers usually involve two steps: RNA-seq reads are first mapped to the reference genome using an aligner, such as BLAT [ 85], TopHat [ 86] or Bowtie [ 87], and then a graph representing all possible isoforms from overlapping reads is constructed, and the isoforms are resolved by traversing the graph.
For reasons described below, a second assembly of the reads was constructed using the Velvet algorithm.
The hybrid-assembly of these reads was constructed using a novel pipeline (see Methods) and with a Newbler assembly of the 454 reads as a starting point.
A set of transcripts consisting of cDNAs from TriFLDB and contigs from RNA-Seq reads was constructed as shown in Figure 1 (steps 1 5).
Besides the assemblies of these two sets of sequences, allowing for direct comparison, a combined transcriptome using both sets of reads was constructed.
The data sets studied in this context comprise 14 original data sets (each consisting of around 16 million reads) and 400 reduced data sets, as ten independent subsamples per data set size (10 million, 4 million, and 1 million reads) were constructed for each taxa (fig. 1).
A "super-read" is constructed from a paired-end read when the ends can be extended uniquely (as judged from the list of k-mers) to form a single uninterrupted sequence containing both reads.
The smoothed sequence read profile is constructed as a superposition of all such Gaussians.
These read sets are constructed by first finding all reads that overlap each particular clone, and then performing intersection and subtraction operations on the sets of reads to isolate smaller regions.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com