Before treatment with test chemicals, basal calcium flux was recorded at 515 575 nm following excitation at 470 495 nm for 100 s in 0.125 s read intervals using the FLIPR tetra plate reader (Molecular Devices).
The sampling to reading interval was not optimal but was the best that could be achieved under very harsh field conditions.
Data acquisition on the FlexStation 3 system (Molecular Devices) was performed at room temperature using a 1.52- s reading interval throughout the experiments.
A final extension was carried out by 95°C for 15 sec followed by 60°C for 1 min. A melting curve was performed from 55 to 95°C with a 0.2°C reading interval.
On the soundtrack, verses from Ben Okri's specially commissioned poem are read at intervals, and the words intimate wide worlds – cosmic space, fertility, growth, decay – that feel too transcendent for the more impressionistic gazing on stage.
A novel may be read at intervals and with interruptions; a play has breaks between the acts; but the short story is rarely put down and in this it resembles the film, which makes a unique demand for uninterrupted attention upon its audience.
The instrument was preheated to 37°C and fluorescence intensity was read at intervals of 15 s.
Apparent viscosities (cP; 1 cP = 1 mPa·s) were read at intervals of 4 seconds for 1 hour, and recorded by the computer.
Incubation was performed at room temperature and the plate was read at intervals between 5 and 30 min up to a period of 24 h.
Incubation was performed at room temperature on a 96-well microplate and the plate was read at intervals between 5 and 30 min up to a period of 24 h.
