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Reader conditions were as follows: 481 nm excitation, 509 nm emission, gain of 50, and read height of 7.0 mm.
The actual focus height had only minimal effect on the detection of spot numbers but we found that the lower the read height, the greater the fluorescence measurement.
Regions with sufficient read coverage were identified using H, L and G parameters, which are read height, length in base pairs and gap permitted in the length parameter, respectively.
The reactions were stopped by the addition of 4 μl of ADP-Glo™ Reagent 1 (Promega) with 40 min incubation, followed by 8 μl of kinase detection reagent for a further 60 min in the dark, before reading plates using a Pherastar plate reader (BMG Labtech) with a luminescence filter, at a read height of 14 mm and a 0.5 s integration time.
Read height and interval.
Similar(55)
Values were obtained through reading peak height with a 10 sec read time.
In cases where these procedures yielded M equivalent locations, we assigned reads of height 1/ M to each location.
As a measure of read through efficacy peak height ratios were determined about 300 bases after and 50 bases before the hairpin.
The police description of the missing woman read: "Aged 35, height 5ft 7in, hair reddish and shingled, eyes grey, complexion fair.
By adjusting the height read of the micrometer, we induced a pressure onto the samples.
As a result of group retardation of the radar wave, a practical approach to infer hmF2 values is to use empirical formulas that connect hmF2 to the maximum usable frequency (MUF) factor, M3000F2 parameter, rather than working with the virtual height read from the ionogram (Bradley and Dudeney, 1973).
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