Following preparation of sufficiently high quality total RNA, samples were labelled, loaded onto the array slides and read following the manufacturer's instructions.
As I have in previous years, here is a not-best-of list, but everything I read following the Manson book, with some stops for thoughts on memorable highlights along the way.
Impression smears from the placenta, were stained with Giemsa and read following standard procedures [19].
Blood slides were Giemsa-stained and read following standard quality-controlled procedures [21].
Results were read following the manufacturer's instructions and interpreted according to MIC interpretive guidelines by Clinical Laboratory Standards Institute.
Tightness in the variation of read length and number of high quality bases per read following trimming indicates a high quality set of reads for downstream analysis (see Figure 2).
Plates were read following overnight incubation at 35 °C.
Fluorescence was read following each annealing and extension phase.
Plates containing oxacillin disk were read following a 24 hour incubation period.
Luminescence was immediately read following the addition of the luciferin solution using a Molecular Devices SPECTRAmax GEMINI.
Blood slides from Guinea and The Gambia were read at the Medical Research Council MRCC) Unit in The Gambia; blood slides from Benin were read, following the same protocol, at Entomological Research Centre of Cotonou.
