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To avoid aliasing, oversampling was performed in read direction (head to foot).
Therefore the FOV was chosen to 300 mm in read direction to cover the entire sand bowl and 37.5 mm in phase encoding direction to guarantee that no body parts of the sandfish fold on each other.
The SNPs at positions 158585487 and 158594972 had a frequency below the cut off for variant calling, but these were investigated in addition to the called SNPs (SNP 158585487 if validated would cause a non-synonymous change that would be potentially damaging, and SNP 158594972 is at a very high frequency only in one read direction).
Two-fold over-sampling was performed in read direction (head/foot direction) to prevent aliasing.
Secondly, we also observed biases that are specific to forward/reverse read direction.
We also observed a poor performance of the F5 sequence read direction.
Similar(36)
But usually, you can read directions and do this yourself.
In Maryland, Andy described his plans to start an online cooking show while Alex read directions from his iPhone.
Does the cook read directions?
I take time to read directions!
Variant calls were then validated by comparison of read directions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com