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We applied our pipeline to the BAM files obtained by mapping the FASTQ-formatted paired-end read data obtained from COLO-829 cell line to the human reference genome using BWA [ 24].
In Table 2, we present the start and end sites of these duplications reported by [ 26] and identified by DB.> We also applied our method to the paired-end read data obtained from the cell line COLO-829, immortalized from a 43-year-old male with metastasis of a malignant melanoma.
Read data obtained from in planta and in vitro experiments were filtered (Table S1) and used for transcriptome assembly.
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Illumina short read sequence data obtained from 101 of the clinical infection isolates (Additional file 1: Table S1) were mapped to the 3D7 P. falciparum reference genome sequence, enabling high quality genome-wide SNP calling and analysis for 85 isolates (45 from Kintampo and 40 from Navrongo) passing the quality filtering described in the Methods (Additional file 1: Table S1).
Oono et al. [ 21] verified the three de novo assembly tools (e.g., Trans-Abyss, Velvet-Oases, and Trinity) by comparing analyses from several programs using short-read sequence data obtained from wheat cultivar CS seedlings grown under reduced phosphorus.
In this study, we verified the de novo assembly approach by comparing analyses from several programs using short-read sequence data obtained from wheat cultivar CS seedlings under –P.
Currently, the following protocol is in use in the CSR project (a BioMuta data source) to identify variations: Short read data are obtained from TCGA (http://cancergenome.nih.gov/) via The Cancer Genomics Hub data portal (https://cghub.ucsc.edu/).
Users can install METAREP and import annotation data obtained from reads or assemblies using METAREP's index generation scripts.
For example in RNA-Seq data obtained from yeast, no reads matching a 3.5-kb deleted region were obtained, and very few reads matching to nontranscribed centromeres were identified [ 32].
Indeed, when eQTL mapping for the YRI was conducted using genotype data obtained from RNA-seq reads (i.e., the same pipeline used for the baboons), we identified 290 genes with eQTL (41.4% of those identified using independently collected genotype data).
Therefore, by subtracting the data obtained from BS-Seq, where C and 5fC read as a U, from data obtained by fCAB-Seq where only C reads as a U and 5fC reads as a C, 5fC can be identified as the difference.
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