The non-redundant set of reads comprised 2,539,403 sequences, and the reads that were sequenced only once (termed here "singlets") comprised 2,167,946 sequences, corresponding only to 9% of the perfect matches (Table 1), suggesting that our sequencing reached a high level of saturation.
They are also very handy in lists, particularly when items consist of several words or contain punctuation themselves: "His holiday reading comprised Eats, Shoots & Leaves Sheffieldd United FC: the Official Centenary History; and Through the Looking-Glass, and What Alice Found There".
Artifactual reads comprised of primer or adapter sequences were also removed at this stage.
It was followed by let-7, miR-31a, miR-275 and miR-276a, with a number of reads comprised between 10,000 and 30,000.
Preprocessing all 324 samples resulted in 511,687 high-quality reads comprised of 10,369 unique sequences.
The mapped reads comprised ∼7.8 Gbp in total and ranged from 887 Mbp to 1.4 Gbp among the seven tissues.
We obtained 502 reads with tags that did not match any of the used tag sequences; these reads comprised 0.33% of all reads.
The un-annotated reads comprised most of the total reads and accounted for 30 ~ 50% of the total reads in the four libraries.
The read that had the same alignment score in the two genomes was discarded by the custom script (such reads comprised a very low percentage).
The data were combined with 98,086 Sanger EST reads comprised of 2 × 43,474 paired reads obtained from both 5′- and 3′-ends of each cDNA clone and 11,138 single-end reads from either end.
