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(1)The user logic waits for a start signal from the user through slave register 0. (2)The different addresses to access the right AIO card fields are set up, namely, the BCR and read buffer.
The plate was then washed three times with PBS/0.05% Tween 20 and 150 µl of 2x read buffer was added to each well.
After washing three times with TBS, 2× read buffer was added to the plate and imaged using a Sector Imager 2400A.
After washing three times with PBS with 0.05% Tween-20, 2× read buffer was added to the plate and imaged using a Sector Imager 2400A.
MSD-T read buffer was added to the plates and signals were detected using Sector Imager 2400 reader (Meso Scale Discovery).
After washing three times with PBS with 0.05% Tween-20, 2× read buffer was added to the plate and imaged using a Sector Imager 2400A (Meso Scale Discovery).
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The cell achieves low power dissipation due to its series connected drivers driven by bitlines and read buffers which offer stack effect.
Note that converting the read buffers to integers is as fast for the base 150 data as for the uncompressed data.
The plate was incubated with 50 µl of streptavidin-phycoerythrin for 35 minutes and then with 120 µl of reading buffer for 3 minutes at room temperature with shaking.
Finally, reading buffer was added and samples were run with Luminex instrument (Bio-Plex™ 200 System, based on Luminex® xMAP Technology).
MCL-1-BIM complexes were detected with rabbit anti-BIM antibody (clone Y36) from Abcam, followed by Sulfo-tagged anti-rabbit antibody (Meso Scale Discovery; cat no. R32AB-5) and Meso Scale Discovery reading buffer with surfactant (cat. no. R92TC-2) according to the manufacturer's recommendations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com