Sentence examples for read alignment to from inspiring English sources

Once CCS reads are sorted, the pipeline uses Bowtie2's default alignment (end-to-end read alignment) to align reads to the corresponding reference sequence and outputs a set of alignments in SAM format.

mRNA abundance is determined by sequencing either 3' end tags or random cDNA fragments (digital transcript expression, DTE), followed by read alignment to reference databases and calculation of aligned read frequencies.

Briefly, the Burrows-Wheeler aligner (BWA version 0.6.1-r104 [ 36]) was used for read alignment to the University of Maryland Bovine reference assembly UMD3.1 build 137 [ 12].

In the case of sample V4528, Mosaik failed to report read alignment to the consensus.

One major issue for all bis-DNA Next Generation sequencing (BisSeq) experiments is read alignment to a reference genome.

Such read alignment to contigs is necessary to provide support for new transcript identification as well as for determining gene expression levels [ 49, 50].

Reference plasmid sequences were closed and corrected with visual inspection of BWA (Burrows-Wheeler aligner -generated Illuminaligner -generatedalIllumina to plandid contigs (28) and annotated with PacBio (29).

Short read alignments to the contig set revealed almost 367,000 genetic polymorphisms segregating in the An.

TopHat uses full-length read alignments to build a set of exon 'islands', then searches for short reads that bridge these exon islands.

The read alignments to different unitigs were converted to distance estimates for the contig assembly stage.

An example of read alignments to precursor miRNA are shown Figure 1A.