The reactive signals were visualized by ECL kit (PE Applied Biosystems, Foster City, USA).
Reactive signals were visualised using the BCIP/NBT Color Development Substrate (Promega).
In addition, nitric oxide (NO) produced in the heart by nitric oxide synthase (NOS) is a highly reactive signaling molecule and an important modulator of cardiac function.
For example, if reactive signals suggest that the ball may not come over the home plate, the fact that it is also the first pitch would make it easier for the brain to decide not to swing the bat.
The immune-reactive signal was visualised using DAB substrate, which stains target proteins yellow.
In non-demented control cases (without AD pathology), a low intense immune-reactive signal of EphA4 was detected in the parenchyma.
Since few cells die in late embryonic stages (3-fold and 4-fold stages), the TUNEL-reactive signals seen in 4-fold nuc-1 mutant embryos must be DNA breaks that were generated during early embryonic stages and persisted through the late embryonic stages [23], [30].
However, the immuno-reactive signals for GABAα1 at the cell surface were comparable in WT and HD brain slices.
The cysteine thiol group is particularly versatile, and the concept has emerged that different thiol-reactive signalling molecules can selectively modulate protein function [ 16].
