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To evaluate membrane integrity, an amine reactive dye was utilized.
The labeling of aaUTP-cRNA was performed by use of Alexa Fluor 555 Reactive Dye (#A32756; LifeTechnologies).
Viable cells were identified using LIVE/DEAD Fixable Dead Cell Stain kit with Aqua fluorescent reactive dye (Life Technologies, USA).
However, the commercial coagulant managed to reduce the COD of the reactive dye by 94% and to remove 96% of the color for the reactive dye.
Additionally, the extracted coagulant was able to reduce the COD of the reactive dye by 73% and to remove 99% of the color of the reactive dye.
To make these labels, the surface of the disc is coated with a reactive dye that changes colour on exposure to the laser light.
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smFISH probes were synthesized by J.M. Escudier (SPCMIB, Toulouse, France) and labeled with Cy3 mono-reactive dye pack (GE Healthcare).
To help prevent premature opening, though, the container has a spot of heat-reactive dye on the label that turns a different color at the proper temperature.
Individual vials of Cy3/Cy5 from the mono-reactive dye packs (GE Healthcare) were dissolved in 200 µl DMSO.
Different concentrations of Alexa Fluor 647 non-reactive dye were prepared through serial dilutions and passed through the flowcell, from which fluorescence intensity measurements were taken.
A-549, HCT-116 and MCF7 cells were grown at 70 80% confluence and cell surface proteins labeled with Cy5 fluorescent mono-reactive dye (Amersham, Uppsala, Sweden).
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