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In this paper we report microwave induced reactive base wash (MRW) as a fast, green alternate to conventional filtrate washing.
While ODN3 has a central 5-IdU, the reactive base in ODN4 is positioned towards the 5′-end of the ODN.
The differences in reactivity and yield between the two strands were small, thus suggesting that the position of the reactive base does not greatly influence the rate of reaction, highlighting the flexibility and generality of the methodology.
Further analysis will be required to determine whether reactive base propenal species modify DNA repair proteins in cells under oxidative stress and to characterize the effects of modification on DNA repair activity in vivo.
The most reactive base propenal, adenine propenal, is mutagenic in Escherichia coli and reacts with DNA to form covalent adducts; however, the reaction of adenine propenal with protein has not yet been investigated.
This formed amide ion is a very reactive base but less so than BuLi that generated it.
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The quantitative shape data were normalized to a scale that falls between 0 for un-reactive sites and reactive bases would attain an average reactivity of 1.
All aggregates tested in this study were considered as reactive based on the above mentioned 14-, 28- and 56-day failure criteria.
Referring to Fanon, this scenario could be described as reactive, based on the continuous non-recognition of the Roma as European citizens.
DHPAA appears to be highly reactive based on its chromatographic behavior (see Fig. 2 & Fig. 4).
Unlike the highly reactive, base-sensitive cyclohexanespiro-3′-oxaziridine 7, the increased steric hindrance of oxaziridine 140 adds significant stability.
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