Exact(10)
DNA templates for in vitro transcription reactions were generated by PCR, with one primer containing the T7 promoter sequence (Table S2).
RT-PCR reactions were generated using the OneStep RT-PCR kit (Qiagen Inc., Mississauga, Canada) with either the universal influenza A generic primer, Uni12, as reported elsewhere [54] or with A/H1N1pdm-specific primers (Table S6).
The sample-specific, palindromic, self-hybridizing barcodes used in the tagging reactions were generated using a self-complementary 8-nt barcode and a rare restriction site according the same protocol.
PCR primers and primer extension probes were designed using SpectroDESIGNER software (Sequenom), and all PCR amplifications and primer extension reactions were generated by PCR-ABI 9700 thermocyclers (Applied Biosystems, Foster City, CA, USA).
Primers used in amplification reactions were generated by Sigma-Genosys (The Woodlands, TX).
Dynamic systems based on consecutive thia-Michael and Henry reactions were generated and transformed using lipase-catalyzed asymmetric transformation.
Similar(50)
Limited spontaneous reactions maintain constant apparent activation energy for the oxidation until the chain reactions are generated; the apparent activation energy then increases.
Additionally, a compact high-temperature n-heptane oxidation model of 47 species and 468 reactions was generated by the application of necessity analysis to the skeletal mechanism.
The corresponding template is extracted, whereafter a set of 25 test reactions is generated.
These test reactions are generated using the database entry from which the template was extracted and the available mapping for that entry.
Reactions are generated by randomly splitting and recombining molecules.
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