This includes the development of study protocols, questionnaires, databases and data entry screens, the data entry, plausibility checks, validation of clinical outcomes, reporting of adverse drug reactions, data analysis, report writing, publications of results, archiving.
Fusion reactions and data analysis were performed as previously described [6], [49].
MLPA reactions and data analysis were performed as previously described [25], [26].
RNA isolation, reverse transcription, qRT-PCR reactions and data analysis were performed as previously described [ 30].
All PCR reactions and data analysis were performed on the iCycler iQ Real-Time Detection System (Bio-Rad).
JDG conceived of the experiments, authored the manuscript, selected lines for analysis, isolated DNA from lines, performed PCR, RT-PCR, cloning, bulk segregant analysis, SSR genotyping, sequencing reactions and data analysis.
The test, designed by GEP faculty, covers basic knowledge of genes and genomes (20-question annotation quiz) and basic knowledge of sequencing reactions and data analysis (25-question finishing quiz) in a multiple-choice format.
PS developed the study design; supervised the preparation and quality assessment of RNAs, and the microarray experiments; participated in performing RT-qPCR reactions and data analysis; drafted the manuscript.
Gene array at 6 hours after CSF1-Fc administration was performed using cytokine and chemokine array RT profiler polymerase chain reaction arrays and analyzed using the online RT profiler polymerase chain reaction array data analysis (version 3.5; Qiagen, UK), and presented by Volcano plot.
The challenge is to keep the processes of sample preparation, sequencing reaction and data analysis balanced.
Regarding reaction times, data analysis revealed that females in the LU group showed faster responses than the other group in affective responsiveness to negative stimuli, specifically sad and angry situations.
