The results of this work confirm that RSM is a reliable tool not only for optimization of the reaction parameters and prediction of GY in RIG processes, but also for the reduction of the number of the experiments, monomer consumption and absorbed dose leading to an improvement of the overall reaction cost.
Commonly, the whole reaction cost about 0.5 h.
The synthesis step and the reaction cost were remarkably decreased.
The estimated reaction cost of microarray assay was eight times lower than that of available commercial methods in Brazil.
Furthermore, the estimated reaction cost was nine times lower than that of available commercial methods in Brazil.
Furthermore, stopping the reaction at 24 h would allow to reduce the reaction cost.
Thus, this multiplexed capture method is highly scalable, substantially lowering the capture reaction cost per sample.
The per reaction cost for the GAS LCGreen assay is low, at approximately $2.40 US, including DNA extraction and PCR reagents and consumables, but not including swab costs and personnel time.
Each reaction costs approximately $20 and takes approximately 40 minutes to complete; therefore, a similar experiment would cost approximately $2880 and take four days to complete.
However, there are negative aspects to therapy with biologic agents, such as opportunistic infections, infusion reactions, high cost, and the fact that there are some patients in whom RA remains active regardless of the use of biologics.
Such reactions will cost you your friendship.
