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Activity assay for immobilized enzyme was performed in the same conditions used for free enzyme, except that the reaction were maintained with stirring, and interrupted by separation of enzyme-immobilized beads from the reaction mixture by filtration in a Buchner funnel before the spectrophotometric readings.
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The reaction was maintained at a constant temperature of 30 °C.
The reaction was maintained for one hour in the dark at room temperature with continuous stirring.
In a nuclear reactor the chain reaction is maintained at a controlled, nearly constant level.
The pH of reaction was maintained by addition of NaOH.
In another type, termed an intermediate reactor, the chain reaction is maintained by neutrons of intermediate energy, and a beryllium moderator may be used.
Because of the characteristics of a sub-critical reaction process, the fission chain reaction is maintained by additional neutrons generated by protons in spallation target.
Then 3.5 μl mix of stop buffer (2.1 μl of 0.1 M EDTA, pH 8.0, 0.42 μl of 0.1 M Tris-HCl, pH 8.0, and NF-water) was added and the reaction was maintained at 50 °C for 15 min.
In addition, we confirmed that MnO2 does not convert to Mn3O4 during the charge/discharge cycles if the redox reaction is maintained within a restricted range where a homogeneous process exists between MnO2 and MnOOH.
The grafting polymerization reaction was maintained for 30 min.
The reaction was maintained at 280 °C with reflux to promote growth of the iron oxide nanoparticles.
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