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A two-step reaction was conducted.
Moreover, the reaction was conducted at a relatively low reaction temperature 200 °C.
The copper-catalyzed azide-alkyne cycloaddition reaction was conducted for 18 h with slow rotation at room temperature.
Even when the reaction was conducted at 0 °C for 30 min, 2 was not obtained (4; 14% yield).
Following this methodology, the reaction was conducted under different flow conditions, pH and H2O2 concentrations.
First, the LAMP reaction was conducted successfully with substitution of dUTP for dTTP.
Each PCR reaction was conducted as triplicate in 20 µl volume using Fast Probe Master Mix (Biotium, 31005) with Bio-Rad CFX Connect™ (Bio Rad, CA, USA).
Fischer-Tropsch synthesis reaction was conducted in a supercritical fluid medium using a fixed bed reactor.
The corresponding reaction was conducted in an aqueous milieu that was feasible due to the water solubility of these derivatives.
Reaction was conducted at 70 °C.
The coupling reaction was conducted at room temperature overnight.
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