Sentence examples for reaction transformation from inspiring English sources
Our follow-up results suggested that PCR determines the final success rate of the whole HTP cloning process (Table 1), whereas other steps, such as BP reaction, transformation, have negligible effects on the final outcome.
Based on the DDBP method, the improved GATEWAY cloning strategy and a robotic platform, we constructed a high throughput (HTP) cloning pipeline, including PCR primer design, PCR, BP reaction, transformation, plating, colony picking and entry clones extraction, which have been successfully applied to 90 C. elegans genes, 88 Brucella genes, and 188 human genes.
After receiving primers for 90 C. elegans, 88 Brucella, and 188 human ORFs in 96-well plate, HTP cloning, including PCR, E-Gel check, BP reaction, transformation, colony picking, cell culture and mini-prep, was performed on our integrated robotic platform.
The SMARTS language is supported well including features such as stereochemistry, component grouping, and atom maps (to match reaction transformations).
What biological interactions correspond to the model relations (reactions, transformations, diffusion)?
BP reaction and transformation of DH5α cells were performed according to the GATEWAY protocols from the manufacturer (Invitrogen).
As a chemical reaction, this transformation is referred to as the Bamberger rearrangement and requires strong acid catalysis.
Despite the short thermal pulse durations, severe coating and substrate degradation processes can occur; these include melting, gas metal reactions, metallurgical transformations, interface reactions, transformational stresses, and thermal stresses.
Fluids may also promote metamorphic reactions, like transformation from spinel peridotite to garnet peridotite.
Following mutagenesis reactions and transformation of E. coli, cultures of single colonies were grown and stored.
Standard molecular techniques were used for DNA isolation, cloning, PCR reactions, ligation, transformation, and sequencing [ 66].
