Conditions for amplification of integrated lentivirus insert from genomic DNA were established by pilot PCR reactions to optimize amounts of genomic DNA, DNA polymerase, and number of PCR cycles for each replicate, cell line, and time point.
We designed and tested loop-mediated isothermal amplification (LAMP) primers and lyophilized reactions to optimize the detection of Zaire Ebola Virus (ZEBOV) and further evolved the LAMP platform to allow room-temperature storage for deployment in resource limited settings.
The here presented approach combines catalysis and chromatographic analysis in a single step and allows to study the kinetics of reactions and to optimize reaction conditions.
The mixing kernel in the model provides freedom to redistribute the reaction mixture along a continuous reaction coordinate to optimize product yield.
The objective of this research was to investigate the kinetics of this reaction and to optimize conditions.
In this study, we use molecular dynamics simulations driven by a collective reaction coordinate to optimize full hydrogen bond networks in a set of protein protein docking solutions.
We postulate the latest developments in engineering carbon feedstock, catalysts, and temperature cum other minor reaction parameters to optimize the CNT yield with desired diameter and chirality.
Table 4 Screening of the reaction conditions to optimize the acetalization process Entry Complex (mol%) MeOH (mL) Temperature (°C) Yield a 1 1 (2) 0.25 r.t.
Results In this study, PSMA-617 was labeled with Lu-177 and Tb-161 using different reaction conditions to optimize radiochemical yield and purity.
