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On the other hand, the composite catalyst 1 wt% Pt/USY-CP814E, physically mixed and loaded with Pt using ion exchange and subsequently deactivated as with the 1 wt% Pt/CP814E catalyst, offered slightly higher activity towards the cracked products along the deactivation run, with the initial conversion of 78 wt% decreasing slightly with TOS to 67 wt% with the reaction terminating after 83 h.
Then 100 μl of reaction terminating solution was added to each well and the plate was shook.
The reaction terminating solution (1M phosphoric acid) was added (100 μL) and the plate was read with a spectrophotometer at 450 nm.
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The reaction terminated with 5 min at 72°C.
Data acquisition began at 70°C and increased incrementally by 0.1°C until the reaction terminated at 96°C [ 26].
Immunocomplex formation was measured using tetra-methylbenzidine solution, and the reaction terminated using 2.5 N sulfuric acid.
The reaction mixture was incubated at 37°C for 4 minutes, and the reaction terminated by the addition of 30 μl 1 M HCL.
Third, the first metabolic ligation reaction terminated when Sub_1 was depleted at about 2900 sec and subsequently, the second metabolic ligation reaction would have terminated when all of Prod_A formed by the first ligation reaction was depleted.
The carbophilic nature of electron deficient group 10 transition metal complexes enables them to behave as capable electrophiles for activating olefins, though the resulting M-alkyl species are often susceptible to reaction-terminating β-hydride elimination (e.g., the Wacker reaction).
Level 4: Because of adverse reactions, terminate this research.
Therefore, when ATP is depleted at about 3600 sec, both the enzymatic ligation reactions and the tRNA charging reactions terminate.
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